Analysis of antioxidant activity on cocktail honey products as female pre-conception supplements

Objective: Cocktail honey is derived from a mixture of honey (trigona sp.), bee bread, and homogeneous royal jelly. The material has a phenolic content rich in antioxidants that are beneficial for women's reproductive health, especially for pre-conception, because it can suppress the content of free radicals in the body. Antioxidants are useful to overcome oxidative damage due to free radicals in the body that prevent various diseases from increasing fertility during pre-conception. Method: This study used the DPPH (2,2-diphenyl-1-picrylhydrazyl) test method using UV–vis spectrophotometry to express the value of free radical reduction activity as IC50 (inhibitory concentration) values. Results: The DPPH test on cocktail honey products obtained an average yield of 4577.7 μg/mL, which was included in the product category was very weak in the antioxidant activity content. Conclusion: The content contained in the honey cocktail contains weak bioactive content by assessing the antioxidant content using DPPH. The difference in the results of antioxidant activity tests using DPPH is caused by the test method and the conditions used in processing, homogeneous ingredients, solvent volume, extraction time, temperature, and pressure in product management. (AU)

Ginger honey affects cortisol, estrogen and glutathione levels; preliminary study to target preconceptional women

Objective: This study was aimed to determine the effect of ginger honey supplementation on cortisol, glutathione, and estrogen levels. The study was conducted on mice that had not yet experienced conception, and prior stress induction was carried out so that they could be continued for human trials at the preconception stage and subjects who experienced mild stress. Method: It was an in vivo study, pretest–posttest control group design. The sample of this study was 2–3 months female Balb/c mice, divided into negative control and ginger honey intervention as much as 28 mg/20 g BW for 14 days—the ELISA method used to examine cortisol hormone, glutathione levels, and estrogen levels. The mice chosen were those that had never experienced conception, and before the intervention, swimming activities were carried out on the mice until they showed symptoms of stress. Results: Results show 42 mg/20 g BW of ginger honey administration for 14 days increased 1.892 ng/dl of cortisol (p = 0.165), increased 2.438 ng/dl of glutathione (p = 0.002), and also increased 22.754 ng/ml estrogen levels in induced stress Balb/c female mice (p = 0.001). Conclusion: Ginger honey did not affect reducing cortisol levels but increasing glutathione and estrogen levels significantly. Ginger honey supplements are the potential to use as complementary therapies. (AU)

Effect of royal jelly to deal with stress oxidative in preconception women: A literature review

Introduction: Oxidative stress that occurs in preconception women can disrupt the reproductive system to cause infertility. The antioxidants contained in royal jelly can overcome oxidative stress due to low antioxidants in the body. The purpose of this study is to examine studies of the content of royal jelly, antioxidant activity, and the effectiveness of royal jelly in dealing with oxidative stress in preconception women. Method: This research method is an electronic database search using keywords according to questions in research from the online library PubMed, content science, and Science Direct. Result: a study review conducted in 6 research journals stated that the use of royal jelly as a supplement containing 10-hydroxy-2-decanoic (10-HDA) increases glutathione levels, as well as lipid peroxidation inhibitors. Conclusion: The effectiveness of royal jelly to overcome oxidative stress in preconception women can be assessed from the content of royal jelly and antioxidant activity that can increase the glutathione levels and inhibit increased lipid peroxidation, which is a sign of oxidative stress. (AU)